• 产品名称: 细胞膜制备
  • 产品编号: JSFW42
  • 上架时间: 2016-06-08
  • 浏览次数: 85
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细胞膜制备服务: 
细胞膜是细胞表面的一层薄膜.细胞膜由磷脂双层和相关蛋白质以及胆固醇和糖脂组成,其化学组成主要是脂类、蛋白质和糖类.细胞膜有重要的生理功能,它既是细胞维持稳定代谢的胞内环境,又能调节和选择物质进出细胞,又负责细胞间的信息交流. 正常的细胞膜上有各种受体或者通道,比如GPCRIon channel,这些蛋白是新药研发的热门靶点。在研究药物与药靶间的相互作用时,细胞膜上的binding实验往往成为研究的第一选择。

科佰生物提供标准的细胞膜制备服务,帮助你加速科研进度,提高科研质量。

Fig 1. 10 ug of membranes prepared from CHO cells stably expressing ADRA1A receptors were incubated with indicated concentrations of [3H]Prazosin in the absence (total binding) or presence of 1000-fold excess unlabeled Prazosin (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.

Fig 2. 10 ug of membranes prepared from CHO cells stably expressing ADRA1A receptors were incubated with indicated concentrations of Phentolamine in the presence of 1nM [3H]Prazosin. Binding was terminated by rapid filtration. Data were fit to one-site competition equation using a non-linear regression method.

服务说明:

1. 针对常规细胞提供细胞膜提取服务,特殊细胞请事先咨询

2. 提取的膜用于binding实验,如其他用途,请事先咨询

3. 提取的膜用蛋白浓度定量,通过Sigmoidal dose-responseSecond order计算浓度,按照公式,即可换算成细胞膜的量;

4. 本服务仅对提取的细胞膜的量做QC,质量需要由binding assay来检测。

提交的产品和资料:

1. 细胞膜;

2. 实验报告;

订购方法:

 如需帮助,请发送邮件至sales@cobioer.com或拨打4008750250,科佰生物的技术支持将竭诚为您服务。

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