• 名称: BT-20(人乳腺癌细胞)
  • 货号: CBP60354
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CBP60354
I. General information 
Synonyms: BT-20
Background: This breast tumor line was established by E.Y. Lasfargues and L. Ozzello in 1958 by isolation and cultivation of cells spilling out of the tumor when it was cut in thin slices. ­
Species: Homo sapiens, human
Tissue: mammary gland/breast
Disease: carcinoma
Gender: female,74 years,Caucasian
Morphology: epithelial
Growth Mode: adherent
DNA Profile: Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 11,14
D5S818: 12
D7S820: 10
THO1: 7,9.3
TPOX: 11
vWA: 16,17
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM+10%FBS+10ug/ml insulin or 1% ITS-G(GIBCO 41400045)
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: Antigen expression: HLA A1, Bw16 (+/-)
Tumor Formation: Yes, in nude mice
(The cells form grade II adenocarcinomas.)
Comments: The cells express the WNT3 and the WNT7B oncogenes [PubMed: 8168088].
A mycoplasma contaminant was discovered and eliminated early in 1972.
Growth of BT-20 cells is inhibited by tumor necrosis factor alpha (TNF alpha).
BT-20 cells are negative for estrogen receptor, but do express an estrogen receptor mRNA that has deletion of exon 5.
For more information, please contact Cobioer (4008-750-250).

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