• 名称: OCI-AML5(人急性髓细胞白血病)
  • 货号: CBP60528
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CBP60528
I. General information 
Synonyms: OCI-AML5
Background: established from the peripheral blood of a 77-year-old man with acute myeloid leukemia (AML M4) in relapse in 1990; the cells are described in the literature to respond to G-CSF, GM-CSF, IL-3, and FLT3 ligand; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to rapid outgrowth of independent cells
Species: human (Homo sapiens)
Tissue: N/A 
Disease: acute myeloid leukemia
Gender: N/A 
Morphology: round cells growing singly or in clumps in suspension; 
Growth Mode: N/A 
Doubling Time: about 30-50 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: 80% alpha-MEM(GIBCO 32561037 )+ 20% FBS + 10 ng/ml GM-CSF
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD4 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, cyCD68 +, HLA-DR +;
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, LDH, NP
Cytogenetics: human hyperdiploid karyotype - 48(44-48)<2n>XY, +1, +8, der(1)t(1;19)(p13;p13)
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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