• 名称: CAL-148(人乳腺癌细胞)
  • 货号: CBP60359
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CBP60359
I. General information 
Synonyms: CAL-148
Background: established from the pleural effusion from a 58-year-old French woman with terminally invasive breast cancer (moderately differentiated ductal adenocarcinoma) after repeated cycles of chemotherapy (including adriamycin and taxol)
Species: human (Homo sapiens)
Tissue: breast  
Disease: breast adenocarcinoma
Gender: N/A 
Morphology: round to epitheloid cells growing as single cells or as clumps either in suspension or, if supplemented with EGF, in monolayers;
Growth Mode: N/A 
Doubling Time: ca. 30-40 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM + 20% FBS+ EGF (0.1 µg/ ml)
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human by cytogenetics
Cytogenetics: human near-diploid karyotype with 16% polyploidy - 45-48<2n>XX, +1, +7, -8, -13, +20, add(1)(p36), der(1;16)(q10;p10), del(2)(q11q2?2), del(13)(q1?1q1?3), der(16)t(1;16)(q12-21 - p11) - resembles originator's unpublished karyotype
Virus   PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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