• 名称: CMK(人原始巨核细胞白血病细胞)
  • 货号: CBP60531
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CBP60531
I. General information 
Synonyms: CMK
Background: established from the peripheral blood of a 10-month-old boy with Down's syndrome and acute megakaryocytic leukemia (AML M7) at relapse in 1985
Species: human (Homo sapiens)
Tissue: N/A 
Disease: acute megakaryocytic leukemia
Gender: N/A 
Morphology: single, multiformed, large cells growing in suspension, sometimes loosely adherent (<3%); 2-3% giant, multinucleated cells;
Growth Mode: N/A 
Doubling Time: ca. 40-50 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD41 +, CD71 +, CD235a +; image
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, NP
Cytogenetics: human flat-moded hypotetraploid karyotype with 8% polyploidy - 85-90<4n>XY, -X, -Y, -2, -3, +5, -6, -6, -8, +11, -15, -15, +16, -17, -19, +21, +22, +7-11mar, add(1)(q31), add(1)(p36), add(3)(q11), del(3)(p14)x2-3, add(5)(q11), add(5)(q13), dup(8)(q11q21), add(8)(q13-21), del(8)(q11), del(9)(p21)x2, add(9)(q11)x2, del(10)(q22q24), der(11;17)(q10;q10), der(11)dup(11)(p13p15)t(5;11)(q11;p15)x1-2, del(11)(q23), add(12)(p13)x2, add(17)(p1?), add(18)(q23)x2-3, add(19)(p13), der(20)t(1;20)(q2?5;q1?2)x2, add(22)(q13) - disomic rearrangement of 20q1 close to common deleted segment, together with additional rearrangements of ch 20 present in markers - matches published karyotype
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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