• 名称: CAL-85-1(人乳腺癌细胞)
  • 货号: CBP60361
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CBP60361
I. General information 
Synonyms: CAL-85-1
Background: established from the relapsing invasive galactophoric breast adenocarcinoma resected from a 35-year-old woman during radiotherapy in 1990; cells were described as expressing the multidrug resistance (MDR) gene
Species: human (Homo sapiens)
Tissue: breast  
Disease: breast adenocarcinoma
Gender: N/A 
Morphology: adherent epithelial-like cells growing in monolayers;
Growth Mode: N/A 
Doubling Time: ca. 70 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin (+)
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human by cytogenetics
Cytogenetics: human flat-moded hypotriploid karyotype with 8% polyploidy - 60(57-65)<3n>XX/XXX, +2, +5, -8, -10, -12, -13, -14, -15, -16, -16, -17, -18, +?20, -21, -22, +2-4mar, +fra, add(X)(q2?), del(X)(q11), add(1)(p34), del(2)(q?11q?21)x1-2, add(2)(p2?3), der(3)?inv(3)(p24q29)add(3)(qter), der(3)del(3)(p13)dup(3)(?q13?q26)del(3)(q26qter), del(3)(q21), add(4)(q2?9), der(4)t(4;9)(p15;?q22), der(6)t(6;12)(q22-24;q13), add(7)(p11), del(7)(q32)x2, der(9)dup(9)(p11p24)add(9)(p24), add(11)(q24-25)x1-2, del(12)(p13), der(15;21)(q10;q10), add(17)(p13), add(17)(p13), add(19)(p13), der(?20)t(17;20)(q12;q13)dup(17)(q1?2q2?3), add(21)(p11)
Virus   PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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