• 名称: COLO-824(人乳腺癌细胞)
  • 货号: CBP60362
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CBP60362
I. General information 
Synonyms: COLO-824
Background: established from the pleural fluid of a 52-year-old woman with breast carcinoma in 1991
Species: human (Homo sapiens)
Tissue: breast 
Disease: breast carcinoma
Gender: N/A 
Morphology: cells grow loosely adherent in monolayers, some cells grow as round cells in clumps in suspension
Growth Mode: N/A 
Doubling Time: ca. 60-80 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+15%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, MDH, NP
Cytogenetics: human highly rearranged hyperdiploid karyotype with 11% polyploidy - 51(48-52)<2n>X, +1, -2, -6, +7, -10, -13, -15, -19, -21, -22, +12mar, del(1)(p12), der(1)t(1;?)(p12;?), der(6)t(1;6)(q25;q12), der(7)t(7;?)(q32/34;?), der(8)t(8;?)(q24;?), der(12)t(12;?)(p13;?), der(15)t(15;?)(p12;?), der(17)t(17;?)(p12;?) - carries small bisatellited marker
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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