• 名称: EFM-19(人乳腺癌细胞)
  • 货号: CBP60363
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CBP60363
I. General information 
Synonyms: EFM-19
Background: established from the pleural effusion of a 50-year-old Caucasian woman with breast carcinoma (ductal type, histological grading II) in 1979
Species: human (Homo sapiens)
Tissue: breast 
Disease: breast carcinoma
Gender: N/A 
Morphology: epitheloid, growing in clusters at first, then as monolayer;
Growth Mode: N/A 
Doubling Time: 50-60 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin -
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, MDH
Cytogenetics: human highly rearranged hypotriploid karyotype with 5.8% polyploidy - 62(58-65)<3n>X, der(X), apparent ch 17 nullisomy and preponderance of marker chromosomes, e.g. add(1)(p11), idel(1)(q11q?22), i(1p), i(2p), add(4)(p11), add(4)(p11), etc. - resembes original description
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
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