• 名称: EFM-192A(人乳腺癌细胞)
  • 货号: CBP60364
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CBP60364
I. General information 
Synonyms: EFM-192A
Background: established from the pleural effusion of the left side from a 46-year-old Caucasian woman with metastasizing breast adenocarcinoma in 1985; sister cell lines EFM-192B and C show differences in molecular aspects; erbB-2 gene is amplified and overexpressed
Species: human (Homo sapiens)
Tissue: breast  
Disease: breast carcinoma
Gender: N/A 
Morphology: epitheloid, adherent growing as monolayer; 
Growth Mode: N/A 
Doubling Time: about 9 days
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Fingerprint: same DNA profile as EFM-192B/EFM-192C using multiplex PCR of minisatellite markers
Species: confirmed as human with IEF of AST, NP, MDH
Cytogenetics: human flat-moded near-triploid karyotype with 20% polyploidy - 68(60-70)<3n>XXXX, +X, -1, -4, +9, -12, -17, -17, -18, -22, +6mar, add(1)(p22), add(1)(p?32), add(2)(q35/37), add(3)(q27/29), i(15q), add(17)(p12), dminx1 present at 38%
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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