• 名称: SNU-475(人肝癌细胞)
  • 货号: CBP60218
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CBP60218
I. General information 
Synonyms: SNU-475
Background: SNU-475 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient prior to cytotoxic therapy.
Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum.
Species: Homo sapiens, human
Tissue: liver
Disease: grade II-IV/V, hepatocellular carcinoma
Gender: male,43 years, Asian
Morphology: epithelial
Growth Mode: adherent, monolayer of diffusely spreading cells
Doubling Time: 66 hrs
DNA Profile: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 12
D5S818: 10,13
D7S820: 7,12
THO1: 7,9
TPOX: 8,9
vWA: 14
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: Blood Type AB; Rh +
Comments: Grossly, the original tumor was massive growth type. Histologically, it was predominantly compact and minor trabecular type.
The cultured cells are multinucleated.
Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization.
HBV genomic RNA was not expressed.
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