• 名称: HuP-T4(人胰腺癌细胞)
  • 货号: CBP60540
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CBP60540
I. General information 
Synonyms: HuP-T4
Background: established from the malignant ascites of a 60-year-old Japanese man with pancreas carcinoma in 1985
Species: human (Homo sapiens)
Tissue: pancreas
Disease: pancreas carcinoma
Gender: N/A 
Morphology: epitheloid cells growing adherently in monolayers;
Growth Mode: N/A 
Doubling Time: about 38 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: MEM + 20% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin (+)
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, MDH
Cytogenetics: human near-triploid karyotype with 11% polyploidy and 1-2 supernumerary dm - 69/70(66-73)<3n>X, ?del(X)/XY, -Y, -2, -4, +6, +7, +8, -9, -9, +11, +16, +mar - inv(3)(p22q24), i(8q), idic(9)(pter), add(17)(p12) - 2 medium size ring ch present at 10%
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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