• 名称: JIMT-1(人乳腺癌细胞)
  • 货号: CBP60378
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CBP60378
I. General information 
Synonyms: JIMT-1
Background: established from the pleural effusion of a 62-year-old woman with ductal breast cancer (grade 3 invasive, T2N1M0) after postoperative radiation in 2003; cell line was described in the literature to carry an amplified HER-2 oncogene and to be insensitive to HER-2-inhibiting drugs, e.g. trastuzumab (Herceptin)
Species: human (Homo sapiens)
Tissue: breast  
Disease: breast carcinoma
Gender: N/A 
Morphology: epithelial-like cells growing in monolayers;
Growth Mode: N/A 
Doubling Time: ca. 30-40 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Fingerprint: fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species: confirmed as human by cytogenetics
Cytogenetics: human flat-moded highly rearranged hyperdiploid karyotype with 6% polyploidy - 46-56<2n>XX, +1, +2, +2, +3, +5, +6, +7, +12, -13, -14, -15, -15, -16, -18, +17, +5mar, add(X)(p22.1), add(1)(q11), del(1)(q32.2), der(1)add(1)(p21)add(1)(q22), der(1)t(1;6)(p11;q1?1)t(6;5)(q25;?p13), add(2)(q11)x2, t(2;3)(q10;p10)add(2)(q25-31), add(3)(p11), der(4)t(?3;4)(?q24;p11), add(5)(q15), der(5)add(5)(p15.3)del(5)(q22), der(5)t(5;8)(p11;p11), add(6)(p11)add(6)(q23), der(7)t(1;7)(p13;q21), der(7)t(7;?12)(p11;p11), add(8)(p11), i(8q) add(9)(p11), der(9)add(9)(p11)add(9)(q23), add(11)(p13), der(11)dup(11)(p11p15)add(11)(p15), der(11)t(1;11)(q24;p12), add(12)(q21), der(12;?15)(p11;q11), add(17)(p11), add(17)(p13), der(17)add(17)(p11)add(17)(q21), inc; unresolved HSR markers present
Virus   PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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