• 名称: Karpas 422(人非霍奇金淋巴瘤)
  • 货号: CBP60629
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CBP60629
I. General information 
Synonyms: KARPAS 422
Background: Established from a pleural effusion of 73 year-old woman diagnosed with B-cell non-Hodgkin lymphoma (intra-abdominal, diffuse large cell lymphoma, refractory, terminal). Carries t(14;18) IGH-BCL2 fusion gene (break point in major breakpoint region, MBR).
Species: human (Homo sapiens)
Tissue: B cell lymphoma
Disease: Human B cell non-Hodgkin lymphoma
Gender: 73 year-old   female
Morphology: Round polygonal cells growing singly or in small clusters in suspension
Growth Mode: Suspension
Doubling Time: N/A
DNA Profile: Amelogenin: X
CSF1PO: 11
D13S317: 9,13
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 8,9.3
TPOX: 8,9
vWA: 14,17
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+20%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: Hyperdiploid with 10% polyploidy
Subculture Routine:  If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube; slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 5 x 100,000 cells/ml in fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days. Once the culture is established the serum concentration can be reduced to 10%.Maintain cultures between 5 - 20 x100,000 cells/ml by splitting saturated cultures 1:2 every 2-4 days; 5% CO2; 37°C. Doubling time approximately 60-90 hours. Round polygonal cells growing singly or in small clusters in suspension.
Comments: For more information, please contact Cobioer (4008-750-250).

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