• 名称: MDA-MB-435S(人乳腺导管癌细胞)
  • 货号: CBP60407
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CBP60407
I. General information 
Synonyms: MDA-MB-435S
Background: This cell line was originally described as a spindle shaped variant of the parental MDA-MB-435 strain isolated in 1976 by R. Cailleau, et al. from the pleural effusion of a 31 year old female with metastatic, ductal adenocarcinoma of the breast.
However, recent studies have generated questions about the origin of the parent cell line, MDA-MB-435, and by extension HTB-129. Gene expression analysis of the cells produced microarrays in which MDA-MB-435 clustered with cell lines of melanoma origin.
Species: Homo sapiens, human
Tissue: previously described as: mammary gland/breast; derived from metastatic site: pleural effusion
Disease: previously described as ductal carcinoma
Gender: female, 31 years,Caucasian
Morphology: spindle shaped
Growth Mode: adherent
Doubling Time: N/A
DNA Profile: Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 13
D5S818: 12
D7S820: 8,10
THO1: 6,7
TPOX: 8,11
vWA: 16,18
Cobioer’s Cell Line Authentication Service
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 2
PGM3, 1
Culture Medium: DMEM + 10% FBS+10 ug/ml insulin+ 10 ug/ml glutathione
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: N/A
Receptor Expression: N/A
Oncogene: N/A
Genes Expressed: tubulin; actin
Cellular Products: tubulin; actin
Tumor Formation: No, in immunosuppressed mice
Yes, in semisolid medium
Comments: This cell line was originally described as a spindle shaped variant of the parental MDA-MB-435 strain isolated in 1976 by R. Cailleau, et al. from the pleural effusion of a 31 year old female with metastatic, ductal adenocarcinoma of the breast.
However, recent studies have generated questions about the origin of the parent cell line, MDA-MB-435, and by extension HTB-129. Gene expression analysis of the cells produced microarrays in which MDA-MB-435 clustered with cell lines of melanoma origin instead of breast [PubMed ID: 10700174, PubMed ID: 15150101, PubMed ID: 15679052].
Additional studies have since corroborated a melanocyte origin of MDA-MB-435, to which ATCC has responded by pursuing its own investigation into the identity of this cell line. The cell line to which MDA-MB-435 is reported to have been cross-contaminated with is the M14 melanoma line [PubMed ID: 12354931 and PubMed ID: 17004106].
Derivatives of HTB-129 with identities in question:
M4A4, ATCC® CRL-2914
M4A4 GFP, ATCC® CRL-2915
M4A4 LM3-2 GFP, ATCC® CRL-2916
M4A4 LM3-4 CL 16 GFP, ATCC® CRL-2917
NM2C5, ATCC® CRL-2918
NM2C5 GFP, ATCC® CRL-2919
For more information, please contact Cobioer (4008-750-250).

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