• 名称: BFTC-905(人膀胱移行细胞癌)
  • 货号: CBP60638
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CBP60638
I. General information 
Synonyms: BFTC-905
Background: established from a 51-year-old Chinese woman with a grade III papillary transitional cell carinoma of the urinary bladder in 1990; patient lived in a blackfoot disease endemic area in Taiwan
Species: human (Homo sapiens)
Tissue: urinary bladder
Disease: urinary bladder transitional cell carcinoma
Gender: N/A 
Morphology: epitheloid cells growing adherently in monolayers, forming large islets; 
Growth Mode: N/A 
Doubling Time: ca. 60-70 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM+15%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays; bacterial contamination was eliminated with Baytril (enrofloxacin) and BM-Cyclin (tiamulin & minocycline)
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin (+)
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, LDH
Cytogenetics: human flat-moded hyperdiploid/hypotriploid karyotype - 58(52-61)<3n>X/XX-X, -4, +5, -9, -13, -15, -16, -18, -21, -22, -22, +mar, add(1)(p36), i(5p), add(5)(q35), del(9)(p22), del(11)(p11), add(11)(q13), add(13)(p12), add(13)(p12), add(15)(p12), add(19)(p13) - i(5p) and loss of 9p associated with transitional cell carcinoma - matches published karyotype
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
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