• 名称: hFOB 1.19(人SV40转染成骨细胞)
  • 货号: CBP60724
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CBP60724
I. General information 
Synonyms: hFOB 1.19
Background: This line was established by transfection of limb tissue obtained from a spontaneous miscarriage with the temperature sensitive expression vector pUCSVtsA58 and the neomycin resistance expression vector pSV2-neo. Clones were selected in the presence of 0.6 mg/mL G418.
Species: Homo sapiens, human
Tissue: bone
Gender: fetus
Morphology: osteoblast; SV40 large T antigen transfected
Growth Mode: adherent
Doubling Time: 36 hours 
DNA Profile: Amelogenin: X
CSF1PO: 10,13
D13S317: 11,12
D16S539: 9,13
D5S818: 11,12
D7S820: 8,10
THO1: 7,9.3
TPOX: 11
vWA: 16,18
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM:F12(1:1)+0.3 mg/ml G418+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: SV40 T antigen
Genes Expressed: alkaline phosphatase
Cellular Products: alkaline phosphatase
Comments: Cells grown at a permissive temperature of 33.5°C exhibit rapid cell division (Doubling time of 36 hrs), whereas little cell division occurs at a restrictive temperature of 39.5°C (Doubling time of 96 hrs).
The cells have the ability to differentiate into mature osteoblasts expressing the normal osteoblast phenotype. At the restrictive temperatures, cell division is slowed, differentiation increases, and a more mature osteoblast phenotype is produced.
For more information, please contact Cobioer (4008-750-250).

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