• 名称: ACHN(人肾腺癌细胞)
  • 货号: CBP60704
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CBP60704
I. General information 
Synonyms: ACHN
Background: The ACHN cell line was initiated in November, 1979 from the malignant pleural effusion of a 22-year-old Caucasian male with widely metastatic renal adenocarcinoma (autopsy confirmed). Cells were seeded directly to culture flasks in Eagle's MEM with 10% FBS, then maintained and passaged 150 days in flasks. Cells were then inoculated subcutaneously into nude mice. After 4 weeks, palpable, locally invasive tumors were noted. Both the original cells (ACHN) and those recovered from nude mouse tumors were growth-inhibited by human interferons.
Species: Homo sapiens, human
Tissue: kidney; derived from metastatic site: pleural effusion
Disease: renal cell adenocarcinoma
Gender: Caucasian,male,22 years 
Morphology: epithelial
Growth Mode: adherent
DNA Profile: Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 12,13
D5S818: 12
D7S820: 9,11
THO1: 8
TPOX: 8,11
vWA: 16,17
Cobioer’s Cell Line Authentication Service
Culture Medium: MEM+10%FBS+1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Oncogene: p53 +; pRB +
Genes Expressed: p53 +; pRB +
Tumor Formation: Yes, in nude mice,(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
Comments: Growth is inhibited by human interferon [Hogan, T. F., personal communication].
For more information, please contact Cobioer (4008-750-250).

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