• 名称: YAPC(人胰腺腺癌细胞)
  • 货号: CBP60698
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CBP60698
I. General information 
Synonyms:  
Background: established from ascites obtained during exploratory laparatomy of a 43-year-old Japanese man with pancreas carcinoma (terminal, no therapy) in December 1993; cells were described as being dependent on autocrinely secreted IL-1alpha
Species: human (Homo sapiens)
Tissue: pancreas
Disease: pancreas carcinoma
Gender: N/A 
Morphology: cobblestone-like adherent cells growing in monolayers; 
Growth Mode: N/A 
Doubling Time: ca. 48 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 (+), cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin (+)
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, NP
Cytogenetics: human near-triploid karyotype with 8% polyploidy - 66-71<3n>XXYY, +3, +7, +8, +9, +10, +14, -17, -18, -19, -20, -22, -22, -22, +3mar, der(1)del(1)(q23-25)inv(1)(p11q21), der(1;5)(q10;q10), add(3)(p11)/del(3)(p11p21)x2, del(5)(p11), add(6)(p21), del(7)(q32), add(9)(p1?), der(9;15)(q10;q10), der(10)t(10;11)(p11;q13)x2, del(12)(p12), add(13)(p11)x2, add(13)(p11), inv(16)(p13q11.2), del(18)(q21), add(20)(p11)/der(20)t(1;20)(p34;p11) - loss of 1p, 3p, 9p, 18q associated with pancreas carcinoma - matches published karyotype
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
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