• 名称: SCC-4(人舌头鳞癌细胞)
  • 货号: CBP60688
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CBP60688
I. General information 
Synonyms: SCC-4
Species: Homo sapiens, human
Tissue: tongue
Disease: squamous cell carcinoma
Gender: male,55 years
Morphology: epithelial-like
Growth Mode: adherent
DNA Profile: Amelogenin: X,Y
CSF1PO: 11
D13S317: 11,13
D16S539: 12
D5S818: 13
D7S820: 9,11
THO1: 9.3
TPOX: 8
vWA: 15,17
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM/F12(1:1)+10%FBS+0.5 mM sodium pyruvate +400 ng/ml hydrocortisone
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Genes Expressed: epidermal keratins; 40 kD keratin
Cellular Products: epidermal keratins; 40 kD keratin
Tumor Formation: Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells.
Comments: SCC-4 forms colonies in semi-solid medium, and is not induced to differentiate by anchorage deprivation.Clonal growth of these cells is improved by using a 3T3  (ATCC CCL-92) feeder layer (see Rheinwald and Green, Cell 6:331, 1975 for methods) Ref.
ATCC grows these cells on 56-X.2, MITC-STO cells. The feeder cells must be plated 24 hours before use at 2 X 106/T75 in order to obtain a 30% confluent monolayer.
For more information, please contact Cobioer (4008-750-250).

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