• 名称: NCI-H1623(人非小细胞肺癌细胞)
  • 货号: CBP60059
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CBP60059
I. General information 
Synonyms: NCI-H1623
Background: The line was established in April 1987.
Species: Homo sapiens, human
Tissue: lung; derived from metastatic site: lymph node
Disease: stage 3B, adenocarcinoma; non-small cell lung cancer
Gender: male, 58 years, Caucasian,The patient was a smoker.
Growth Mode: adherent, single cells and loosely attached clusters
Culture Medium:
DMEM: F12(1:1)+5%FBS:
• 0.02 mg/ml insulin
• 0.01 mg/ml transferrin
• 25 nM sodium selenite (final conc.)
• 50 nM Hydrocortisone (final conc.)
• 1 ng/ml Epidermal Growth Factor (do not filter)
• 0.01 mM ethanolamine (final conc.)
• 0.01 mM phosphorylethanolamine (final conc.)
• 100 pM triiodothyronine (final conc.)
• 0.5% (w/v) bovine serum albumin (final conc.)
• 10 mM HEPES
• 0.5 mM sodium pyruvate (final conc.)
• extra 2mM L-glutamine (for final conc. of 4.5mM)
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Comments: For more information, please contact Cobioer (4008-750-250).

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