• 名称: CAL-12T(人非小细胞肺癌细胞)
  • 货号: CBP60050
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CBP60050
I. General information 
Synonyms: CAL-12T
Background: established from a male patient with a non-small cell lung carcinoma in 1981
Species: human (Homo sapiens)
Tissue: lung
Disease: non-small cell lung carcinoma
Gender: N/A 
Morphology: adherent epithelial-like cells growing in monolayers; image; image
Growth Mode: N/A 
Doubling Time: ca. 50 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP +, neurofilament -, vimentin -
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, NP
Cytogenetics: human hypotriploid karyotype with 12% polyploidy - 59(57-59)<3n>X, +1, -2, -4, -5, -6, -6, -7, -11, -12, -13, -16, -16, -18, +20, +4-5mar, der(1;18)(p10;q10), der(1)t(X;1)(?;p?21)t(1;?)(q1?1;?), der(1)(q12->q10::p10->pter), add(3)(p11), add(4)(p11), add(5)(p15), add(9)(q1?)x1-2, add(11)(q24), dup(11)(q13q24), del(12)(p12), i(14q), add(19)(q11), i(21q)
Virus   PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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