• 名称: LOU-NH91(人肺癌鳞癌细胞)
  • 货号: CBP60081
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CBP60081
I. General information 
Synonyms: LOU-NH91
Background: established from the lower right lung lobe removed two months after diagnosis of highly differentiated squamous cell lung carcinoma with metastasis to one lymph node (no visceral involvement) in a 64-year-old woman (without any prior therapy)
Species: human (Homo sapiens)
Tissue: lung  
Disease: lung squamous cell carcinoma
Gender: N/A 
Morphology: adherent ovoid-to-epitheloid cells growing in monolayers; image; image
Growth Mode: N/A 
Doubling Time: ca. 80 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640 + 20% FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, LDH, NP
Cytogenetics: human hyperdiploid karyotype with declining hyperhaploid sideline (2%) and 9% polyploidy - 50-53<2n>XX/XXXX, +5, +5, +7, +7, -13, +17, -18, +22, +22, +1-2mar, del(4)(q27), ?add(22)(q13)x2 (major clone) - 54<2n>XXXX, +5, +5, +7, +7, +22, +22 (minor clone) - 27XX, +5, +7, +22 - matches published karyotype - unstable karyotype with recent gain of structural rearrangements
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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