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科研细胞

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T84(人结肠癌细胞)

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产品编号
CBP60021
货号:
CBP60021
品牌:
Cobioer
规格:
T-25 Flask
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CBP60021
I. General information 
Synonyms: T84
Background: The T84 cell line is a transplantable human carcinoma cell line derived from a lung metastasis of a colon carcinoma in a 72-year-old male. Tumor tissue was inoculated subcutaneously and serially transplanted in BALB/c nude mice.
Species: Homo sapiens, human
Tissue: colon; derived from metastatic site: lung
Disease: colorectal carcinoma
Gender: male,72 years
Morphology: epithelial
Growth Mode: adherent
DNA Profile: Amelogenin: X
CSF1PO: 10
D13S317: 9
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 6,9
TPOX: 8,11
vWA: 17,18
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM/F12(1:1) + 10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Receptor Expression: neurotransmitter, expressed
peptide hormone, expressed
Genes Expressed: carcinoembryonic antigen (CEA), 600 ng/mL per 106 cells per 10 days; keratin
Cellular Products: carcinoembryonic antigen (CEA), 600 ng/ml per 10 exp6 cells per 10 days; keratin
Tumor Formation: Yes, in nude mice,(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
Comments: The original histological characteristics of the colon carcinoma were maintained throughout transplantation in nude mice. After 23 passages in athymic mice, the T84 cell line was established. These cells grow to confluence as monolayers and exhibit tight junctions and desmosomes between adjacent cells.
They have receptors for many peptide hormones and neurotransmitters and maintain vectorial electrolyte transport.This line exhibits tight junctions, and desmosomes between adjacent cells. The cells are positive for keratin by immunoperoxidase staining.
For more information, please contact Cobioer (4008-750-250).
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