|I. General information|
|Background:||This line was isolated in 1975 by T.U. Semple, et al. from ascitic fluid of a 70-year-old Caucasian male with carcinoma of the colon. The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen.|
|Species:||Homo sapiens, human|
|Tissue:||colon; derived from metastatic site: ascites|
|Disease:||Dukes' type D, colorectal adenocarcinoma|
|Gender:||male, 70 years, Caucasian|
|Growth Mode:||mixed, adherent and suspension|
|DNA Profile:||Amelogenin: X
Cobioer’s Cell Line Authentication Service
We strongly suggest to purchase the complete medium from Cobioer.
|Genes Expressed:||carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10),The cells are positive for keratin by immunoperoxidase staining.|
|Cellular Products:||carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10)|
|Tumor Formation:||Yes, in nude mice,(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)|
|Comments:||The line was derived from tissue from the same patient as COLO 201 (ATCC CCL-224). The cells are CSAp negative (CSAp-).
The cells are positive for keratin by immunoperoxidase staining. COLO 205 cells express a 36000 dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen.
For more information, please contact Cobioer (4008-750-250).