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SW620(人结肠腺癌细胞)

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SW620(人结肠腺癌细胞)

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产品编号
CBP60036
货号:
CBP60036
品牌:
Cobioer
规格:
T-25 Flask
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CBP60036
I. General information 
Synonyms: SW620
Background: SW620 was initiated by A. Leibovitz, et al., from a lymph node in the same manner as was the primary adenocarcinoma from which SW480 was derived the previous year.It was isolated from the tissue of a 51-year-old Caucasian male (blood group A, Rh+) as was SW480 (ATCC CCL-228). The line was derived from a metastasis of the same tumor from which the SW480 (ATCC CCL-228) was derived.
Species: Homo sapiens, human
Tissue: colon; derived from metastatic site: lymph node
Disease: Dukes' type C, colorectal adenocarcinoma
Gender:  male, 51 years,Caucasian
Morphology: epithelial
Growth Mode: adherent
DNA Profile: Amelogenin: X
CSF1PO: 13,14
D13S317: 12
D16S539: 9,13
D5S818: 13
D7S820: 8,9
THO1: 8
TPOX: 11
vWA: 16
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: Blood type A; Rh+
Oncogene: myc +; myb + ; ras +; fos +; sis +; p53 +; abl -; ros -; src -
Genes Expressed: Carcinoembryonic antigen (CEA) 0.15 ng/106 cells/10 days; transforming growth factor alpha; matrilysin. The cells are negative for expression of CSAp (CSAp-) and colon antigen 3, negative. The cells are positive for keratin by immunoperoxidase staining. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.
Tumor Formation: Yes, in nude mice,(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cell
Comments: A recurrence of the malignancy resulted in a wide-spread metastasis from the colon to an abdominal mass.The established cell line consists mainly of individual small spherical and bipolar cells lacking microvilli. The cells synthesize only small quantities of carcinoembryonic antigen (CEA), and are highly tumorigenic in nude mice.
The cells are negative for expression of CSAp (CSAp-) and colon antigen 3, negative.The cells are positive for keratin by immunoperoxidase staining. The line was derived from a metastasis of the same tumor from which the SW480 (ATCC CCL-228) was derived.There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.N-myc oncogene expression was not detected.
For more information, please contact Cobioer (4008-750-250).
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