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NCI-H23(人非小细胞肺癌腺癌细胞)

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NCI-H23(人非小细胞肺癌腺癌细胞)

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产品编号
CBP60132
货号:
CBP60132
品牌:
Cobioer
规格:
T-25 Flask
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CBP60132
I. General information 
Synonyms: NCI-H23
Background: This line was derived from a lung cancer obtained from a patient prior to therapy.
Species: Homo sapiens, human
Tissue: lung
Disease: adenocarcinoma; non-small cell lung cancer
Gender: male,  51 years, Black
Morphology: epithelial
Growth Mode: adherent
Doubling Time: 38 hrs
DNA Profile: Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 11
D5S818: 12,13
D7S820: 9,10
THO1: 6
TPOX: 8,9
vWA: 16,17
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Oncogene: myc +; src +; abl +; erb +; ras +; sis -
Genes Expressed: myc +; src +; abl +; erb +; ras +; sis -
Comments: The cells carry the K-ras 12 mutation, and there is a mutation in codon 246 (ATC -> ATG, isoleucine -> methionine) of the p53 gene. There is expression of C-myc, L-myc, v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras and N-ras RNAs. The cells express heterogeneous mRNA expression for PDGF A and B chain, transforming growth factor alpha and beta and the epidermal growth factor receptor (EGFR).
NCI-H23 exhibits a high degree of c-myc DNA amplification (20-fold) but no detectable amplification of c-myc RNA. The cells stain positive for keratins 5+8 and 18 and vimentin but are negative for neurofilament. NCI-H23 cells are L-dopa decarboxylase-negative. They have a reported colony forming efficiency of 9.7% in soft agarose.
For more information, please contact Cobioer (4008-750-250).
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