|I. General information|
|Background:||This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a bone marrow metastasis taken from a patient prior to therapy.|
|Species:||Homo sapiens, human|
|Tissue:||lung; Derived from metastatic site: bone marrow|
|Disease:||stage E,carcinoma; variant small cell lung cancer|
|Gender:||male，55 years adult，Caucasian|
|Growth Mode:||round clusters in suspension|
|Doubling Time:||36 hrs|
|DNA Profile:||Amelogenin: X,Y
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|Receptor Expression:||insulin-like growth factor II (IGF II); bombesin|
|Oncogene:||myc +; myb +; fes +; fms +; raf +; ras +|
|Tumor Formation:||Yes, the cells produce tumors in athymic nude mice|
|Comments:||NCI-H526 expresses elevated levels of 2 biochemical markers of SCLC: neuron specific enolase and the brain isoenzyme of creatine kinase. They do not express L-dopa carboxylase or bombesin-like immunoreactivity. These cells express the c-kit gene as well as the N-myc gene, but not c-myc or L-myc. N-myc is amplified and p75 c-myb expression was observed.
NCI-H526 also expresses the proto-oncogenes N-ras, Ki-ras, Ha-ras, and c-raf1. Only trace amounts of the retinoblastoma susceptibility gene, RB mRNA were detected.RB protein was not detected. The cells express easily detectable levels of p53 mRNA compared to levels found in normal lung.Abnormally sized mRNA was present.The line has a reported colony-forming efficiency of 4.2% in soft agarose.
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