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NCI-H526(人小细胞肺癌细胞)

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NCI-H526(人小细胞肺癌细胞)

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产品编号
CBP60141
货号:
CBP60141
品牌:
Cobioer
规格:
T-25 Flask
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CBP60141
I. General information 
Synonyms: NCI-H526
Background: This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a bone marrow metastasis taken from a patient prior to therapy.
Species: Homo sapiens, human
Tissue: lung; Derived from metastatic site: bone marrow
Disease: stage E,carcinoma; variant small cell lung cancer
Gender: male,55 years adult,Caucasian
Morphology: epithelial
Growth Mode: round clusters in suspension
Doubling Time: 36 hrs
DNA Profile: Amelogenin: X,Y
CSF1PO: 11
D13S317: 13
D16S539: 9
D5S818: 11
D7S820: 9,12
THO1: 6,8
TPOX: 8,11
vWA: 16,17
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Receptor Expression: insulin-like growth factor II (IGF II); bombesin
Oncogene: myc +; myb +; fes +; fms +; raf +; ras +
Tumor Formation: Yes, the cells produce tumors in athymic nude mice
Comments: NCI-H526 expresses elevated levels of 2 biochemical markers of SCLC: neuron specific enolase and the brain isoenzyme of creatine kinase. They do not express L-dopa carboxylase or bombesin-like immunoreactivity. These cells express the c-kit gene as well as the N-myc gene, but not c-myc or L-myc. N-myc is amplified and p75 c-myb expression was observed.
NCI-H526 also expresses the proto-oncogenes N-ras, Ki-ras, Ha-ras, and c-raf1. Only trace amounts of the retinoblastoma susceptibility gene, RB mRNA were detected.RB protein was not detected. The cells express easily detectable levels of p53 mRNA compared to levels found in normal lung.Abnormally sized mRNA was present.The line has a reported colony-forming efficiency of 4.2% in soft agarose.
For more information, please contact Cobioer (4008-750-250).
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