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SHP-77(人小细胞肺癌细胞)

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SHP-77(人小细胞肺癌细胞)

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产品编号
CBP60151
货号:
CBP60151
品牌:
Cobioer
规格:
T-25 Flask
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CBP60151
I. General information 
Synonyms: SHP-77
Background: It was established in 1977 by Edwin R. Fisher and John D. Paulson.,The line was derived from a non-encapsulated primary lung tumor from the apical portion of the upper lobe of the left lung.
Species: Homo sapiens, human
Tissue: lung
Disease: carcinoma; small cell lung cancer
Gender: male,54 years,Caucasian
Morphology: epithelial
Growth Mode: suspension and loosely adherent
Doubling Time: 96 hrs
DNA Profile: Amelogenin: X
CSF1PO: 10,11
D13S317: 8
D16S539: 11
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 9,11
vWA: 16
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: Blood Type O; Rh +; CD56; CD57 (HNK-1,Leu-7)
Genes Expressed: neural cell adhesion molecule (NCAM) NKH-1
Cellular Products: neural cell adhesion molecule (NCAM) NKH-1
Tumor Formation: Yes, the cells form tumors in athymic nude mice, and usually grow as circumscribed nodules without evidence of metastases
Comments: The SHP-77 (Shadyside Hospital, Pittsburgh, PA) cell line is a biochemically stable continuously cultured cell line which has retained important features of SCLC. This cell line is an unusual undifferentiated large cell variant of small cell lung carcinoma.
It has the morphology of a variant, but the biochemical properties of a classic SCLC.Electron microscopy revealed the presence of gland formation and intracytoplasmic lamellar bodies.The cells have neuroendocrine markers L-dopa decarboxylase and dense core secretory granules. SHP-77 can serve as an in vitro target in 51Cr and 111In release cytotoxicity assays as well as in vivo nude mice assays for evaluating immune reactivity of cells and serum from lung cancer patients.The cells can be used to evaluate the immune status of patients with SCLC who are treated with radiation or chemotherapy.
For more information, please contact Cobioer (4008-750-250).
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