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U-937(人组织细胞淋巴瘤细胞)

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U-937(人组织细胞淋巴瘤细胞)

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产品编号
CBP60277
货号:
CBP60277
品牌:
Cobioer
规格:
T-25 Flask
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CBP60277
I. General information 
Synonyms: U-937
Background: The U-937 cell line was derived by Sundstrom and Nilsson in 1974 from malignant cells obtained from the pleural effusion of a patient with histiocytic lymphoma.
Species: Homo sapiens, human
Tissue: Pleura/pleural effusion, lymphocyte, Myeloid
Disease: histiocytic lymphoma
Gender: male,37 years,Caucasian
Morphology: monocyte
Growth Mode: suspension
DNA Profile: Amelogenin: X
CSF1PO: 12
D13S317: 10,12
D16S539: 12
D5S818: 12
D7S820: 9,11
THO1: 6, 9.3
TPOX: 8,11
vWA: 14, 15
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Receptor Expression: complement (C3)
Genes Expressed: lysozyme; beta-2-microglobulin (beta 2 microglobulin); tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid (PMA)
Cellular Products: lysozyme; beta-2-microglobulin (beta 2 microglobulin); tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid (PMA)
Comments: Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures,
The cells are negative for immunoglobulin production and Epstein-Barr virus expression.
The cells express the Fas antigen, and are sensitive to TNF and anti-Fas antibodies.
In 1994, PCR and cytogenetic analyses showed that a number of stocks of U-937 were contaminated with the human myeloid leukemia cell line, K-562.
In the earliest stocks available, the level of contamination was 0.6%.
Distribution was discontinued in March 1994, except if required for patent purposes.
Anyone who wishes to receive a sample of this original material should contact the Head of the ATCC Patent Depository.
A stock of CRL-1593 found to be free of K-562 was propagated continuously for 8 weeks and tested weekly by PCR.
Distribution and seed stocks give DNA profiles characteristic of U-937 only.
Such preparations are now offered as authentic U-937 (ATCC CRL-1593.2) and are believed to be free of second subpopulations.
For more information, please contact Cobioer (4008-750-250).
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