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LN229(人脑胶质细胞瘤)

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LN229(人脑胶质细胞瘤)

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产品编号
CBP60302
货号:
CBP60302
品牌:
Cobioer
规格:
T-25 Flask
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CBP60302
I. General information 
Synonyms: LN229
Background: The LN-229 cell line was established in 1979 from cells taken from a patient with right frontal parieto-occipital glioblastoma [PubMed. 10416987].
Species: Homo sapiens, human
Tissue: brain/right frontal parieto-occipital cortex
Disease: glioblastoma
Gender: female,60 years,White
Morphology: epithelial
Growth Mode: adherent
DNA Profile: Amelogenin: X
CSF1PO: 12
D13S317: 10,11
D16S539: 12
D5S818: 11,12
D7S820: 8,11
THO1: 9.3
TPOX: 8
vWA: 16,19
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM + 10% FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Oncogene: p53 + (mutated, CCT (Pro) --> CTT (Leu) mutation at codon 98), PTEN + (wild type), p16 - (deleted), p14ARF - (deleted)
Tumor Formation: Yes, forms tumors in nude mice
Comments: The cells exhibit mutated p53 (TP53) and possible homozygous deletions in the p16 and p14ARF tumor suppressor genes. They have a wild-type PTEN gene.
Stimulation of the cells with Fas ligand lead to apoptotic cell death within 16 hours. The cells were also killed by puromycin in a dose dependent manner.
Bcl-2 protects these cells from Fas ligand-induced cell death but was shown to have only a small protective effect on puromycin-induced apoptosis.
For more information, please contact Cobioer (4008-750-250).
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