LNCap clone FGC（人前列腺癌细胞）
|I. General information|
|Synonyms:||LNCap clone FGC|
|Background:||LNCaP clone FGC was isolated in 1977 by J.S. Horoszewicz, et al., from a needle aspiration biopsy of the left supraclavicular lymph node of a 50-year-old Caucasian male (blood type B+) with confirmed diagnosis of metastatic prostate carcinoma.|
|Species:||Homo sapiens, human|
|Tissue:||prostate; derived from metastatic site: left supraclavicular lymph node|
|Gender:||male，50 years adult， Caucasian|
|Growth Mode:||adherent, single cells and loosely attached clusters|
|Doubling Time:||about 34 hours|
|DNA Profile:||Amelogenin: X,Y
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|Receptor Expression:||androgen receptor, positive; estrogen receptor, positive
|Genes Expressed:||human prostatic acid phosphatase; prostate specific antigen|
|Cellular Products:||human prostatic acid phosphatase; prostate specific antigen|
|Tumor Formation:||Yes, in soft agar
Yes, the cells are tumorigenic in nude mice
|Comments:||These cells are responsive to 5-alpha-dihydrotestosterone (growth modulation and acid phosphatase production).
The cells do not produce a uniform monolayer, but grow in clusters which should be broken apart by repeated pipetting when subcultures are prepared.
They attach only lightly to the substrate, do not become confluent and rapidly acidify the medium.
Growth is very slow.
The cells should be allowed to incubate undisturbed for the first 48 hours after subculture.
When flask cultures are shipped, the majority of the cells become detached from the flask and float in the medium. Upon receipt, incubate the flask (in the usual position for monolayer cultures) for 24 to 48 hours to allow the cells to re-attach. The medium can then be removed and replaced with fresh medium.
If desired, the contents of the flask can be collected, centrifuged at 300 X g for 15 minutes, resuspended in 10 mL of medium and dispensed into a single flask.
For more information, please contact Cobioer (4008-750-250).