|I. General information|
|Background:||Epithelial cells derived from the peripheral zone of a histologically normal adult human prostate were transfected with a single copy of the human papilloma virus 18 (HPV-18) to establish the RWPE-1 (ATCC CRL-11609) cell line. Ref|
|Species:||Homo sapiens, human|
|Gender:||male， 54 years adult，Caucasian, White|
|DNA Profile:||Amelogenin: X,Y
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|Culture Medium:||K-SFM Basal Medium + Keratinocyte-SFM Growth Supplement (GIBCO 10744-019)+BPE(LONZA生产，科佰分装)
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|Antigen Expression:||kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens, expressed (upon exposure to androgen),Ref (upon exposure to androgen)|
|Receptor Expression:||androgen receptor, expressed , Ref (upregulated upon exposure to androgen)|
|Genes Expressed:||cytokeratin 18, cytokeratin 8, Tumor Supressor Gene(s): p53 +, pRB + Ref|
|Cellular Products:||cytokeratin 18 cytokeratin 8|
|Tumor Formation:||No, in nude mice (with or without Matrigel) Ref No, in soft agar Ref|
|Comments:||In 3-dimensional Matrigel culture, RWPE-1 cells organize into acini and secrete PSA into the lumen when exposed to androgen. Ref
When injected with Matrigel or with stromal cells, into male athymic rodents, RWPE-1 cells also organize into acini Ref and produce PSA.
Cells from the RWPE-1 cell line were further transformed by Ki-ras using the Kirstin murine sarcoma virus (Ki-MuSV) to establish the tumorigenic RWPE-2 cell line (ATCC CRL-11610) Ref and the RWPE2-W99 (ATCC CRL-2853) cell line.
Further, a family of tumorigenic cell lines, that mimics multiple steps in prostate cancer progression, was also derived from RWPE-1 cells by exposure to N-methyl-N-nitrosourea (MNU). See the WPE1-NA22 (ATCC CRL-2849), WPE1-NB14 (ATCC CRL-2850, WPE1-NB11 (ATCC CRL-2851) and WPE1-NB26 (ATCC CRL-2852) cell lines.
The depositor reports that the RWPE-1 cell line (ATCC CRL-11609) was screened, and found negative for, Hepatitis B virus, Hepatitis C virus and Human immunodeficiency virus. ATCC confirmed this cell line is positive for the presence of HPV viral DNA sequences via PCR.
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