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CAL-51(人乳腺癌细胞)

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CAL-51(人乳腺癌细胞)

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产品编号
CBP60360
货号:
CBP60360
品牌:
Cobioer
规格:
T-25 Flask
物种:
组织:
乳腺
数量
-
+
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0
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CBP60360
I. General information 
Synonyms: CAL-51
Background: established from the pleural effusion metastasis of a 45-year-old woman with progressive breast adenocarcinoma (after radio-, chemotherapy and surgery) in 1985; rare example of tumor cell line with normal karyotype
Species: human (Homo sapiens)
Tissue: breast  
Disease: breast carcinoma
Gender: N/A 
Morphology: epithelial-like adherent cells growing in monolayers; the culture contains usually a large amount of cell debris;
Growth Mode: N/A 
Doubling Time: 50-60 hours
DNA Profile:

Amelogenin: X

CSF1PO: 11,12

D3S1358: 16,17

D5S818: 12,13

D7S820: 7,12

D8S1179: 14

D13S317: 11,13

D16S539: 11,13

D18S51: 15,17

D21S11: 31,32

FGA: 22,23

Penta D: 10,14

Penta E: 7,12

TH01: 7

TPOX: 8,9

vWA:18
Cobioer’s Cell Line Authentication Service

Culture Medium: MEM+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, LDH, NP
Cytogenetics: human near-diploid karyotype with 28% tetraploidy - 46<2n>XX, no consistent abnormality detected - rare example of tumor cell line with normal karyotype - resembles published karyotype
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).
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