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MCF 10A(人正常乳腺上皮细胞)

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MCF 10A(人正常乳腺上皮细胞)

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产品编号
CBP60419
货号:
CBP60419
品牌:
Cobioer
规格:
T-25 Flask
物种:
组织:
乳腺
数量
-
+
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CBP60419
I. General information 
Synonyms: MCF 10A
Background: MCF 10A was derived from adherent cells in the population.
Species: Homo sapiens, human
Tissue: mammary gland; breast
Disease: fibrocystic disease
Gender: female, female, Caucasian
Morphology: epithelial
Growth Mode: adherent
Doubling Time: N/A
DNA Profile: Amelogenin: X
CSF1PO: 10,12
D13S317: 8,9
D16S539: 11,12
D5S818: 10,13
D7S820: 10,11
THO1: 8,9.3
TPOX: 9,11
vWA: 15,17
Cobioer’s Cell Line Authentication Service
Isoenzymes: AK-1, 1
Ref
ES-D, 1
Ref
G6PD, B
Ref
GLO-I, 1-2
Ref
PGM1, 1-2
Ref
PGM3, 1
Ref
Culture Medium: MEGM+100 ng/ml cholera toxin
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: N/A
Receptor Expression: N/A
Oncogene: N/A
Genes Expressed: N/A
Cellular Products: N/A
Tumor Formation: No, in immunosuppressed mice
Ref
Yes, in semisolid medium
Ref
Comments: The MCF 10A cell line is a non-tumorigenic epithelial cell line.
The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen.
They exhibit three dimensional growth in collagen, and form domes in confluent cultures.
Thus far, the cells have shown no signs of terminal differentiation or senescence.
The line is responsive to insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor (EGF).
By electron microscopy the cells display characteristics of luminal ductal cells but not of myoepithelial cells.
They also express breast specific antigens as detected by positive reaction with MFA-Breast and MC-5 monoclonal antibodies.
The calcium content of the medium exerts a strong effect on the morphology of the cells.
For more information, please contact Cobioer (4008-750-250).
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