|I. General information|
|Background:||This line was derived from BALB/c mouse xenografts, initiated with EpH4 cells stably transfected with an expression vector containing Glu-Glu epitope-tagged phosphorylation site MEK1 mutant (MEKDD). Cells were rederived from the primary mammary fat pad tumors after selection with 1 mg/mL G418 in complete medium.|
|Species:||Mus musculus, mouse|
|Tissue:||breast (mammary gland)|
|DNA Profile:||Cobioer’s Cell Line Authentication Service|
|Culture Medium:||DMEM+10% FBS
We strongly suggest to purchase the complete medium from Cobioer.
|Oncogene:||Constitutively activated form of MEK1, expressed|
|Genes Expressed:||Glu-Glu tagged phosphorylation site MEK1 mutant (MEKDD), verified at ATCC|
|Cellular Products:||stably expresses Glu-Glu tagged MEKDD, which is a constitutively activated form of MEK1 (Asp218/Asp222 MEK1 phosphorylation site mutant)|
|Comments:||This line was derived from BALB/c mouse xenografts, initiated with EpH4 cells stably transfected with an expression vector containing Glu-Glu epitope-tagged phosphorylation site MEK1 mutant (MEKDD). Cells were rederived from the primary mammary fat pad tumors after selection with 1 mg/mL G418 in complete medium.
Activation of MEK1 is mediated through phosphorylation of Ser218 and Ser222 by members of the Raf family of kinases.
This cell line stably expresses constitutively activated form of MEK1 (MEKDD), and can be used in MEK-MAPK pathway studies and breast tumor in vivo studies. This cell line is tumorigenic, and can be used as a breast cancer in vivo model
This line is one of four related cell lines: B-MEKDD 116 cell line (ATCC CRL-3069), EpH4 1424.1 cell line (ATCC CRL-3209), EpH4 1424.2 cell line (ATCC CRL-3210) and EpH4-Ev cell line (ATCC CRL-3063).
The EpH 1424 cell line produces the constitutively activated MEK1 mutant MEKDD which has been tagged with Glu-Glu, verified at ATCC.
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