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科研细胞
药靶细胞
标准品
NGS靶向捕获探针
Cal27(人舌头鳞癌细胞)
CBP60427 | |
I. General information | |
Synonyms: | Cal27 |
Background: | Cal 27 was established in 1982 by J. Gioanni (Centre Antoine Lacassagne, Nice Cedex, France) from tissue taken prior to treatment from a 56 year old Caucasian male with a lesion of the middle of the tongue. |
Species: | Homo sapiens, human |
Tissue: | tongue |
Disease: | squamous cell carcinoma |
Gender: | male, 56 years, Caucasian |
Morphology: | epithelial |
Growth Mode: | adherent |
Doubling Time: | 35 hrs |
DNA Profile: | Amelogenin: X CSF1PO: 10,12 D13S317: 10,11 D16S539: 11,12 D5S818: 11,12 D7S820: 10 THO1: 6,9.3 TPOX: 8 vWA: 14,17 Cobioer’s Cell Line Authentication Service |
Culture Medium: |
DMEM+10%FBS Cal27完全培养基,# CBP60427M |
Cryopreservation medium: | 90%FBS+10%DMSO |
Antigen Expression: | N/A |
Receptor Expression: | N/A |
Oncogene: | N/A |
Genes Expressed: | N/A |
Cellular Products: | N/A |
Tumor Formation: | Yes, solid tumors developed within 6 weeks in nude mice inoculated with 2 x 106 cells subcutaneously |
Comments: | CAL 27 cells are epithelial, polygonal with a highly granular cytoplasm. Immunocytochemical studies show strong positive staining with anti keratin antibodies. The cells do not grow well in semi-solid medium. Marked inhibition of thymidine incorporation was observed in the presence of VP16 (etoposide), CCNU (1-[2-chloroethyl]-3-cyclohexyl-1-nitrosourea), VM26 (teniposide), ADM (adriamycin), CPA (cyclophosphamide), and MTX (methotrexate). CAL 27 cells were resistant to treatment with VDS (vindesine sulfate), CDP (cis-platinum) or ACTD (actinomycin D). A culture submitted to the ATCC in December 1993 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. For more information, please contact Cobioer (4008-750-250). |
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