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23132/87(人胃癌细胞)

科研细胞

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23132/87(人胃癌细胞)

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产品编号
CBP60475
货号:
CBP60475
品牌:
Cobioer
规格:
T-25 Flask
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CBP60475
I. General information 
Synonyms: 23132/87
Background: established from the primary tumor of a 72-year-old man with gastric adenocarcinoma in 1987
Species: human (Homo sapiens)
Tissue: gastric  
Disease: gastric adenocarcinoma
Gender: N/A 
Morphology: adherent epithelial cells growing as confluent monolayers;
Growth Mode: N/A 
Doubling Time: about 50 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -; (initial data published by the originators showed negativity, we found the cells to express cytokeratin-7)
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, MDH
Cytogenetics: human hypertetraploid karyotype with 12% polyploidy - 47(45-52)<2n>XY, +20, t(1;15)(p11;p11), t(6;12) (p21;q21), i(13q), t(13;14)(p10;q10) - closely resembles published karyotype
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).
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