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MEC-1(人白血病细胞)

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MEC-1(人白血病细胞)

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产品编号
CBP60514
货号:
CBP60514
品牌:
Cobioer
规格:
T-25 Flask
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CBP60514
I. General information 
Synonyms: MEC-1
Background: established in 1993 from the peripheral blood of a 61-year-old Caucasian man with chronic B cell leukemia (B-CLL in prolymphocytoid transformation to B-PLL); serial sister cell line of MEC-2 (DSMZ ACC 500)
Species: human (Homo sapiens)
Tissue: N/A 
Disease: chronic B cell leukemia
Gender: N/A 
Morphology: round to polymorphic cells growing in suspension, singly or partly in small aggregates, a few cells are slightly adherent;
Growth Mode: N/A 
Doubling Time: ca. 40 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: IMDM+20%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +; image
Fingerprint: same DNA profile as MEC-2 using multiplex PCR of minisatellite markers
Species: confirmed originally as human by cytogenetics
Cytogenetics: human near-diploid karyotype with 10% polyploidy - 46(44-47)<2n>XY, -2, +7, -12, +1-2mar, t(1;6)(q22-23;p21), add(7)(q11), der(10)(10pter->q22::?::2q11->qter), del(17)(p11) - small acf/mar present in most cells - resembles published karyotype
Virus   PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).
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