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MV-4-11(人急性单核细胞白血病细胞)

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MV-4-11(人急性单核细胞白血病细胞)

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产品编号
CBP60522
货号:
CBP60522
品牌:
Cobioer
规格:
T-25 Flask
物种:
组织:
外周血
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CBP60522
I. General information 
Synonyms: MV-4-11
Background: The MV4-11 cell line was established by Rovera and associates from the blast cells of a 10-year-old male with biphenotypic B-myelomonocytic leukemia. The growth factor, granulocyte/macrophage colony-stimulating factor (GM-CSF), was required to establish this cell line and growth factors are necessary for its continuous proliferation in chemically defined medium [PubMed: 3496132].
Species: Homo sapiens, human
Tissue: peripheral blood
Disease: biphenotypic B myelomonocytic leukemia
Gender: male,10 years
Morphology: lymphoblast
Growth Mode: suspension
DNA Profile: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 8,9.3
TPOX: 8,11
vWA: 14,15
Cobioer’s Cell Line Authentication Service
Culture Medium: IMDM+20%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: CD4; Homo sapiens. CD10; Homo sapiens.  CD15, human; Homo sapiens .  CD4 (40-96%); CD10 (4-11%); CD15 (96-99%)
Genes Expressed: CD4; Homo sapiens, CD10; Homo sapiens, CD15, human; Homo sapiens, CD4 (40-96%); CD10 (4-11%); CD15 (96-99%)
Comments: This line can be propagated in medium supplemented with 10% FBS without the addition of growth factors. IL-3 can independently support the long-term growth of this cell line, but IL-3 antagonized the proliferation of MV4-11 cells in the presence of GM-CSF when both factors were used at very low concentrations. Granulocyte colony stimulating factor (G-CSF) synergized with GM-CSF in inducing proliferation of MV4-11 cells; G-CSF alone causes a transient stimulation of the cell line.
This line was formerly designated ATCC HTB-189.Over 96% of these cells are positive by indirect immunofluorescence for the myelomonocytic antigen CD15, 40-96% are positive for the monocytic antigen CD4, and 4-11% are positive for CD10 [PubMed: 3500218].
For more information, please contact Cobioer (4008-750-250).
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