|I. General information|
|Background:||The MV4-11 cell line was established by Rovera and associates from the blast cells of a 10-year-old male with biphenotypic B-myelomonocytic leukemia. The growth factor, granulocyte/macrophage colony-stimulating factor (GM-CSF), was required to establish this cell line and growth factors are necessary for its continuous proliferation in chemically defined medium [PubMed: 3496132].|
|Species:||Homo sapiens, human|
|Disease:||biphenotypic B myelomonocytic leukemia|
|DNA Profile:||Amelogenin: X,Y
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|Antigen Expression:||CD4; Homo sapiens. CD10; Homo sapiens. CD15, human; Homo sapiens . CD4 (40-96%); CD10 (4-11%); CD15 (96-99%)|
|Genes Expressed:||CD4; Homo sapiens, CD10; Homo sapiens, CD15, human; Homo sapiens, CD4 (40-96%); CD10 (4-11%); CD15 (96-99%)|
|Comments:||This line can be propagated in medium supplemented with 10% FBS without the addition of growth factors. IL-3 can independently support the long-term growth of this cell line, but IL-3 antagonized the proliferation of MV4-11 cells in the presence of GM-CSF when both factors were used at very low concentrations. Granulocyte colony stimulating factor (G-CSF) synergized with GM-CSF in inducing proliferation of MV4-11 cells; G-CSF alone causes a transient stimulation of the cell line.
This line was formerly designated ATCC HTB-189.Over 96% of these cells are positive by indirect immunofluorescence for the myelomonocytic antigen CD15, 40-96% are positive for the monocytic antigen CD4, and 4-11% are positive for CD10 [PubMed: 3500218].
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