|I. General information|
|Background:||The cell line was established from the peripheral blood of an acute myeloid leukemia (AML) patient.|
|Species:||Homo sapiens, human|
|Disease:||Acute myeloblastic leukemia|
|DNA Profile:||D5S818: 9,11
Cobioer’s Cell Line Authentication Service
We strongly suggest to purchase the complete medium from Cobioer.
|Comments:||This is a leukemic cell line with an 8;21 chromosome translocation. This translocation juxtaposes the AML1 with ETO (or MTG8) gene, giving rise to the fusion gene AML1-ETO (also known as AML1-MTG or RUNX1-CBF2T1), hence the cells produce chimeric AML1-ETO protein. This protein down-regulates CEBPA mRNA, protein and DNA binding activity, which is crucial for the differentiation of granulocytes.The cells are positive for myeloperoxidase showing a morphology of myeloid maturation.
In proliferation assay the cells in culture showed response to interleukin-3 (IL-3), IL-6, granulocyte colony-stimulating factor (G-CSF), and granulocytemacrophage CSF (GM-CSF), but not to IL-1 or IL-5.
Neither granulocytic nor eosinophilic maturation was observed in the in vitro liquid culture by the addition of dimethyl sulfoxide, G-CSF, or IL-5, respectively. Induction of macrophagelike cells was seen by the addition of phorbol ester. Proliferation is inhibited by 1,25S-(OH)2-16,23-diene-26-F3-10-nor D3.
For more information, please contact Cobioer (4008-750-250).