|I. General information|
|Background:||C3H/10T1/2, Clone 8 was isolated by C. Reznikoff, D. Brankow and C. Heidelberger in 1972 from a line of C3H mouse embryo cells.|
|Species:||Mus musculus, mouse|
|DNA Profile:||Cobioer’s Cell Line Authentication Service|
|Culture Medium:||MEM+10%FBS+1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)
We strongly suggest to purchase the complete medium from Cobioer.
|Tumor Formation:||No, in immunosuppressed mice，RefRef Yes, in semisolid medium，RefRef|
|Comments:||The cells are very sensitive to post confluence inhibition of cell division, do not produce tumors in syngeneic mice, have no background of spontaneous transformation, nor do they contain overt endogenous transforming murine leukemia or sarcoma viruses.The cells are contact sensitive.There is no detectable background spontaneous transformation.They are highly susceptible to transformation by chemical agents.
Tested and found negative for ectromelia virus (mousepox).
Note: the inoculation density, feeding and harvesting schedules must be followed rigidly if the line is to retain its essential characteristics. The batch of serum used for growth and for transformation assays may affect both the morphology of this line and the results obtained.Monolayers established and maintained for the standard transformation assay should be free of all foci after 6 weeks.The depositor recommends that the line be used between the 5th and 15th passages only.
For more information, please contact Cobioer (4008-750-250).