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LN-18(人脑胶质母细胞瘤)

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LN-18(人脑胶质母细胞瘤)

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产品编号
CBP60595
货号:
CBP60595
品牌:
Cobioer
规格:
T-25 Flask
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CBP60595
I. General information 
Synonyms: LN-18
Background: The LN-18 cell line was established in 1976 from cells taken from a patient with a right temporal lobe glioma. 
Species: Homo sapiens, human
Tissue: brain/cerebrum; right temporal lobe
Disease: grade IV,glioblastoma; glioma
Gender: White,male,65 years
Morphology: epithelial
Growth Mode: adherent
DNA Profile: Amelogenin: X,Y
CSF1PO: 12
D13S317: 12,13
D16S539: 11,13
D5S818: 11,13
D7S820: 8,10
THO1: 9
TPOX: 8
vWA: 17,18
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: HLA A2, A9, B5, BW35, DRW3,Ref
Oncogene: p53 + (mutated, TGT (Cys) --> TCT (Ser) mutation at codon 238), PTEN + (wild type), p16 - (deleted), p14ARF - (deleted) Ref
Genes Expressed: fibronectin,Ref
Cellular Products: fibronectin,Ref
Tumor Formation: Yes, forms tumors in nude mice
Comments: The cells are negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells exhibit mutated p53 (TP53) and possible homozygous deletions in the p16 and p14ARF tumor suppressor genes. They have a wild-type PTEN gene.
Stimulation of the cells with Fas ligand lead to apoptotic cell death within 16 hours. The cells were also killed by puromycin in a dose dependent manner.Bcl-2 protects these cells from Fas ligand-induced cell death but was shown to have only a small protective effect on puromycin-induced apoptosis.
For more information, please contact Cobioer (4008-750-250).
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