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JVM-3(人慢性髓细胞样白血病细胞)

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JVM-3(人慢性髓细胞样白血病细胞)

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产品编号
CBP60618
货号:
CBP60618
品牌:
Cobioer
规格:
T-25 Flask
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CBP60618
I. General information 
Synonyms: JVM-3
Background: established from the peripheral blood of a 73-year-old man with B-prolymphocytic leukemia (PLL) at diagnosis; cell line was established by EBV-transformation during treatment with phorbol ester TPA; cells express mRNA of the proto-oncogenes BCL2 and BCL3
Species: human (Homo sapiens)
Tissue: N/A 
Disease: chronic B cell leukemia
Gender: N/A 
Morphology: lymphoblastoid cells growing singly or in clumps in suspension; 
Growth Mode: N/A 
Doubling Time: ca. 30 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; 
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: originally confirmed as human with IEF of AST, MDH, NP, later by DNA-fingerprinting
Cytogenetics: human near tetraploid karyotype without clear mode - 87-93<4n>XXYY, +3, -7, -8, -10, +12, +12, -14, -19, -19, -20, -20 - appears to be derivate of original hyperdiploid line which exhibited trisomy 12
Virus   ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).
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