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Farage(人非霍奇金淋巴瘤细胞)

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Farage(人非霍奇金淋巴瘤细胞)

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产品编号
CBP60668
货号:
CBP60668
品牌:
Cobioer
规格:
T-25 Flask
物种:
组织:
淋巴
数量
-
+
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0
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CBP60668
I. General information 
Synonyms: Farage
Background: The Farage cell line was adapted to culture in 1990 from a lymph node biopsy of a patient with diffuse large cell non-Hodgkin's lymphoma (DLCL).
Species: Homo sapiens, human
Tissue: derived from metastatic site: lymph node
Disease: non-Hodgkin's B cell lymphoma
Gender: White,female,adult
Morphology: lymphoblast
Growth Mode: suspension
Doubling Time: 24 to 36 hrs
DNA Profile: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,13
D16S539: 11,12
D5S818: 12
D7S820: 12
THO1: 8,9
TPOX: 9
vWA: 14,15
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS+0.05 mM 2-mercaptoethanol
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: CD10 +/-; CD11a + (LFA-1); CD19 +; CD20 +; CD21 +; CD22 +; CD23 +; CD29 (VLA-4) +; CD38 +; CD39 +; CD40 +; CD44 +; CD54 + (ICAM-1); CD58 + (LFA-3); CD23 -; HLA DR +
Comments: The cells do not express surface or cytoplasmic immunoglobulin.Exposure of Farage cells to phorbol 12-myristate 13-acetate (PMA) down-regulated CD21 and CD23 expression.hey do not express the terminal deoxynucleotydyl transferase gene (TdT), nor the recombination activating genes RAG-1 and RAG-2, known as markers of the pre-B cell stage. These results show that Farage represents a mature B-cell rather than a pre-B cell.
Exposure to IL-4 augmented the concentrations of CD23, CD54, and CD58 but diminished the expression of CD21, CD22, and CD38. Incubation with IL-4 for 6 to 8 days led to increased expression of CD11a, CD39, CD40, and to disappearance of CD21 and CD38.The cells are positive for Epstein-Barr virus (EBV).
mature B cel
For more information, please contact Cobioer (4008-750-250).
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