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A3(人T淋巴细胞白血病细胞)

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A3(人T淋巴细胞白血病细胞)

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产品编号
CBP60703
货号:
CBP60703
品牌:
Cobioer
规格:
T-25 Flask
物种:
组织:
血液
数量
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CBP60703
I. General information 
Synonyms: A3
Background: The A3 subclone was derived from a Jurkat cell line obtained from the laboratory of Gerald Crabtree at Stanford University.
Species: Homo sapiens, human
Disease: acute T cell leukemia
Gender: male
Morphology: lymphoblast
Growth Mode: suspension
DNA Profile: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 17,18
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640 +10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Comments: The Jurkat cells were treated with Fas Antibody and isolated by limiting dilution to obtain a cell line that had a low spontaneous rate of resistance to Fas-medicated apoptosis.The resulting wild-type A3 subclone is very sensitive to Fas-mediated apoptosis.Wild-type A3 cells were made neomycin resistant and treated with three cycles of exposure to the frameshifting mutagen ICR-191 to isolate clones harboring recessive mutations that were resistant to killing by Fas antibody.
ICR-191 treated clones were serially diluted in 96-well plates in the presence of Fas Antibody for 3 to 5 weeks.Two of these ICR-191 treated clones have been deposited at the ATCC. They are I 9.2 (ATCC CRL-2571), a clone with a mutation in the cysteine protease caspase-8/FLICE and I 2.1 (ATCC CRL-2572), a clone with a mutation in the adaptor FADD.
For more information, please contact Cobioer (4008-750-250).
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