南京科佰生物科技有限公司官方网站欢迎您的光临！
免费咨询热线： 4008-750-250
科研细胞
药靶细胞
标准品
NGS靶向捕获探针
hFOB 1.19（人SV40转染成骨细胞）
| CBP60724 | |
| I. General information | |
| Synonyms: | hFOB 1.19 |
| Background: | This line was established by transfection of limb tissue obtained from a spontaneous miscarriage with the temperature sensitive expression vector pUCSVtsA58 and the neomycin resistance expression vector pSV2-neo. Clones were selected in the presence of 0.6 mg/mL G418. |
| Species: | Homo sapiens, human |
| Tissue: | bone |
| Gender: | fetus |
| Morphology: | osteoblast; SV40 large T antigen transfected |
| Growth Mode: | adherent |
| Doubling Time: | 36 hours |
| DNA Profile: | Amelogenin: X CSF1PO: 10,13 D13S317: 11,12 D16S539: 9,13 D5S818: 11,12 D7S820: 8,10 THO1: 7,9.3 TPOX: 11 vWA: 16,18 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM:F12(1:1)+0.3 mg/ml G418+10%FBS hFOB 1.19完全培养基，# CBP60724M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | SV40 T antigen |
| Genes Expressed: | alkaline phosphatase |
| Cellular Products: | alkaline phosphatase |
| Comments: | Cells grown at a permissive temperature of 33.5°C exhibit rapid cell division (Doubling time of 36 hrs), whereas little cell division occurs at a restrictive temperature of 39.5°C (Doubling time of 96 hrs). The cells have the ability to differentiate into mature osteoblasts expressing the normal osteoblast phenotype. At the restrictive temperatures, cell division is slowed, differentiation increases, and a more mature osteoblast phenotype is produced. For more information, please contact Cobioer (4008-750-250). |
扫一扫，关注我们公众号
Copyright ©2021 www.cobioer.com All Rights Reserved. 南京科佰生物科技有限公司版权所有 苏ICP备13038923号