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RKO-E6(结肠癌细胞系)

科研细胞

科研细胞

药靶细胞

药靶细胞

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RKO-E6(结肠癌细胞系)

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产品编号
CBP60741
货号:
CBP60741
品牌:
Cobioer
规格:
T-25 Flask
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CBP60741
I. General information 
Synonyms: RKO-E6
Background: The RKO-E6 cell line was generated from the colon carcinoma RKO (ATCC CRL-2577) cell line by transfection with pCMV-E6 using Lipofectin.
Species: Homo sapiens, human
Tissue: colon
Disease: Carcinoma, Papilloma­
Morphology: epithelial
Growth Mode: adherent
Culture Medium: MEM+10%FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Comments: The cells contain a stably integrated human papilloma virus (HPV) E6 oncogene under control of the cytomegalovirus (CMV) promoter. The HPV E6 oncogene causes a decrease in normal p53 levels and functions. The RKO-E6 cell line lacks appreciable functional p53.   Disruption of normal p53 function in human colon carcinoma RKO cells with the human papillomavirus E6 oncoprotein results in reduced repair of u.v.-induced DNA damage and also loss of induced repair following cellular u.v.-irradiation.
Disruption of normal p53 function in human colon carcinoma RKO cells with the human papillomavirus E6 oncoprotein results in reduced repair of u.v.-induced DNA damage and also loss of induced repair following cellular u.v.-irradiation. The RKO-E6 cell line can be used together with its parental cell line, RKO (ATCC CRL-2577) to investigate the effects of p53 loss on cellular parameters such as p53 mediated transcription and apoptosis.
For more information, please contact Cobioer (4008-750-250).
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