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TF1(人红系白血病细胞)

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TF1(人红系白血病细胞)

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产品编号
CBP60808
货号:
CBP60808
品牌:
Cobioer
规格:
T-25 Flask
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CBP60808
I. General information 
Synonyms: TF1
Background: The TF-1 cell line was established by T. Kitamura, et al. in October 1987 from a heparinized bone marrow aspiration sample from a 35 year old Japanese male with severe pancytopenia. ­
Species: Homo sapiens, human
Tissue: bone marrow
Disease: erythroleukemia
Gender: male,35 years, Japanese
Morphology: lymphoblast
Growth Mode: suspension
Doubling Time: 22 hours
DNA Profile: Amelogenin: X,Y
CSF1PO: 13,14
D13S317: 8,9
D16S539: 9,12
D5S818: 13
D7S820: 12
THO1: 7,9
TPOX: 8
vWA: 15,17
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+2 ng/ml recombinant human GM-CSF+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Comments: The cells are completely dependent on interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for long term growth.
The cells DO NOT RESPOND to interleukin 5 (IL-5). TF-1 cells respond to a variety of other lymphokines and cytokines such as interleukin 1 (IL-1), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 9 (IL-9),Interleukin 11 (IL-11), interleukin 13 (IL-13), stem cell factor (SCF), leukemia inhibitory factor (LIF) and nerve growth factor (NGF). TF-1 cells do not express glycophorin A or carbonyl anhydrase I.
The morphological and cytochemical features, and the constitutive expression of globin genes, indicate the commitment of the cells to the erythroid lineage. Hemin and delta-aminolevulinic acid induce hemoglobin synthesis, and TPA induces dramatic differentiation of the TF-1 cells into macrophage-like cells.
The TF-1 cell line is unique because of its responsiveness to multiple cytokines.
For more information, please contact Cobioer (4008-750-250).
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