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FL 62891(人肝细胞(SV40转化))

科研细胞

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FL 62891(人肝细胞(SV40转化))

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产品编号
CBP60816
货号:
CBP60816
品牌:
Cobioer
规格:
T-25 Flask
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CBP60816
I. General information 
Synonyms: FL 62891
Background: The cell line was derived from human fetal liver tissue, and immortalized by transformation with an ecotropic retrovirus containing a temperature sensitive SV40 T-antigen (tsA58) and a G418 resistance gene. After several days at 37C, the temperature of the medium was lowered to 32C. Cells were selected with G418. The selected cells were expanded and maintained. The cells produce flk-1 and some flk-2 ligands.
Species: Homo sapiens, human
Gender: fetus
Morphology: fibroblast
Growth Mode: adherent
Doubling Time: 48 hours
Culture Medium: IMDM+10%FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Comments: The cell line was derived from human fetal liver tissue, and immortalized by transformation with an ecotropic retrovirus containing a temperature sensitive SV40 T-antigen (tsA58) and a G418 resistance gene. After several days at 37C, the temperature of the medium was lowered to 32C. Cells were selected with G418. The selected cells were expanded and maintained. The cells produce flk-1 and some flk-2 ligands.
For more information, please contact Cobioer (4008-750-250).
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