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Hs68(人男性Canavan病包皮细胞系)

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Hs68(人男性Canavan病包皮细胞系)

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产品编号
CBP60823
货号:
CBP60823
品牌:
Cobioer
规格:
T-25 Flask
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其他
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CBP60823
I. General information 
Synonyms: Hs68
Background: Hs68 is one of a series of human foreskin fibroblast lines developed at the Naval Biosciences Laboratory (NBL) in Oakland, CA. The material was obtained from an apparently normal Caucasian newborn male in February, 1969.
Species: Homo sapiens, human
Tissue: skin; foreskin
Disease: aspartoacylase deficiency; possible Canavan disease
Gender: male,newborn, Caucasian
Morphology: fibroblast
Growth Mode: adherent
DNA Profile: Amelogenin: X,Y
CSF1PO: 9,12
D13S317: 11,13
D16S539: 11,12
D5S818: 8,11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11
vWA: 16
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Comments: Longevity studies carried out at NBL demonstrated that the cells could be propagated for 42 passages.
The ATCC has been informed by Dr. Anne B. Johnson, Department of Pathology and Neuroscience at Albert Einstein College of Medicine, that this cell line has an unusually low level of the enzyme aspartoacylase (0.028 mU/mg or less on repeat assays.
Our records indicate that the donor of Hs 68 was apparently normal, but Dr. Johnson pointed out that individuals exhibiting a deficiency in aspartoacylase suffer from Canavan disease. Canavan disease is a devastating, autosomal recessive disorder involving spongy degeneration of the nervous system.According to Dr. Johnson, fibroblasts from affected children have aspartoacylase levels of 0.003 to 0.02 mU/mg; carriers' levels vary from 0.016 to 0.04 mU/mg and normal run from 0.1 to 0.4 mU/mg. More information on aspartoacylase and Canavan disease can be found in an article by R. Kaul et al.
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