|I. General information|
|Background:||Hs68 is one of a series of human foreskin fibroblast lines developed at the Naval Biosciences Laboratory (NBL) in Oakland, CA. The material was obtained from an apparently normal Caucasian newborn male in February, 1969.|
|Species:||Homo sapiens, human|
|Disease:||aspartoacylase deficiency; possible Canavan disease|
|DNA Profile:||Amelogenin: X,Y
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|Comments:||Longevity studies carried out at NBL demonstrated that the cells could be propagated for 42 passages.
The ATCC has been informed by Dr. Anne B. Johnson, Department of Pathology and Neuroscience at Albert Einstein College of Medicine, that this cell line has an unusually low level of the enzyme aspartoacylase (0.028 mU/mg or less on repeat assays.
Our records indicate that the donor of Hs 68 was apparently normal, but Dr. Johnson pointed out that individuals exhibiting a deficiency in aspartoacylase suffer from Canavan disease. Canavan disease is a devastating, autosomal recessive disorder involving spongy degeneration of the nervous system.According to Dr. Johnson, fibroblasts from affected children have aspartoacylase levels of 0.003 to 0.02 mU/mg; carriers' levels vary from 0.016 to 0.04 mU/mg and normal run from 0.1 to 0.4 mU/mg. More information on aspartoacylase and Canavan disease can be found in an article by R. Kaul et al.
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